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N. Pawar Poonam ¹, P. Chintawar Pavan ¹, Harde Minal ¹, L. Ingale Pramod ², D. Chaudhari Praveen ¹

Affiliations
1 Modern College of Pharmacy, Yamunanagar, Nigdi, Pune 411044, Maharashtra, IN
2 Marathwada Mitra Mandal’s College of Pharmacy, Thergaon, Pune-411 033, Maharashtra, IN

Abstract

Three simple, accurate, economical and reproducible spectrophotometric methods have been developed for the simultaneous estimation of Gatifloxacin (GTX) and Ketorolac Tromethamine (KTA) in ophthalmic dosage form. The first method is Q analysis method based on absorbance ratio at two selected wavelengths 303 nm (iso-absorptive point) and 292 nm (λ_max of Gatifloxacin). The second method is Simultaneous equation method based on measurement of absorbance at two wavelength 292 (λ_max of Gatifloxacin) and 319 (λ_max of Ketorolac Tromethamine) and the third method is Area under curve method and wavelength ranges selected were 290-294 nm (λ₁-λ₂) for Gatifloxacin and 317-321 nm (λ₃-λ₄) for Ketorolac Tromethamine. The linearity lies between 1-12 μg/ml for (GTX) and 1-12 μg/ml for KTA for all the three methods. The results of the analysis were validated statistically and recovery studies were carried out as per ICH guideline. As the % RSD was found less than 2, all the methods were proved to be precise and accurate and can be successfully applied for the simultaneous determination of both the drugs in bulk and ophthalmic dosage form.

Keywords

Gatifloxacin, Ketorolac Tromethamine, Q-Absorbance Ratio Method, Simultaneous Equation Method, Area under Curve Method.

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A. Phatak Atul ¹, D. Chaudhari Praveen ¹

Affiliations
1 Department of Pharmaceutics, P. E. S. Modern College of Pharmacy, Sector-21, Yamunanagar, Nigdi, Pune- 411044 M. S, IN

Abstract

The aim of the present study is to formulate nanosized dispersion of aceclofenac by emulsion-solvent diffusion method and then incorporate it into a gelling agent to produce nanogel. The formulations are characterized for the particle size and found to be below 400 nm. The drug content in the formulations was found satisfactory. DSC and FTIR analysis was performed to ensure the compatibility of the drug and excipients. The optimization was carried out by using factorial design and the formulations are characterized for in vitro drug diffusion, skin permeability, rheological properties and in vivo animal study. The in vivo animal studies showed significant improvement in the activity for the formulation in comparison with the conventional formulation. The formulation showed optimal permeability properties, stability and possessed a sustained drug release during the study period. The nanogel formulation showed the promising alternative to oral administration for aceclofenac.

Keywords

Nanogel, Transdermal Delivery, NSAID, In Vivo Studies.

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R. Balap Aishwarya ¹, V. Prasad Deepshikha ¹, S. Khidse Anuja ¹, B. Jadhav Shailaja ¹, S. V. Joshi ², D. Chaudhari Praveen ¹

Affiliations
1 PES’s Modern College of Pharmacy, Yamunanagar, Nigdi, Pune-411044, IN
2 PES’s Modern College of Pharmacy [For Ladies], Borhadewadi, Dehu-Alandi Road-Moshi, Pune-412105, IN

Abstract

Three simple, precise and economical UV methods have been developed for the determination of Granisetron hydrochloride in pure and it's pharmaceutical dosage forms. Granisetron hydrochloride has the absorbance maxima at 302 nm (Method A) in phosphate buffer (pH 7.0). The quantitative determination of the drug was carried out using the first order derivative, values measured at 241.5 nm (Method B) and Area under Curve (AUC) for analysis in the wavelength range of 299-305 nm (Method C). Calibration graph constructed at 302 nm was linear in concentration range of 2-10 μg/ml with correlation coefficient 0.9994. The proposed methods were successfully applied for the determination of Granisetron hydrochloride in commercial tablet preparation. Results of the analysis were validated statistically and by recovery studies and were found to be satisfactory.

Keywords

Granisetron Hydrochloride, Absorbance Maxima, Derivative Spectrophotometry, Area under Curve.

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